A near infrared fluorescent probe based on ICT for monitoring mitophagy in living cells
Abstract: Mitophagy, a process of cells degrade dysfunctional organelles and recycle their nutrient substance by lysosome, which plays a vital role in cells metabolism and physiology. In this article we presented a highly target and near-infrared (NIR) mitochondria fluorescent probe, which can monitoring the process of autophagy. The response mechanism of probe based on Intramolecular charges transfer (ICT) for process of autophagy detection and living cells real-time imaging. We designed a primary amine as a pH sensitizing group, due to the process of ICT, the probe exhibit green fluorescence, when it was protonated and the process of ICT is broken, the NIR fluorescence will be restored. Simultaneously, the green fluorecence of probe is disappeared. The probe exhibits an excellent selectivity, high sensitivity and clean responsiveness, which indicating that it can be a high-target and high-sensitive imaging of the process of autophagy in living systems.