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A novel colorimetric and far-red emission ratiometric fluorescent probe for the highly selective and ultrafast detection of hypochlorite in water and its application in bioimaging

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Abstract

Hypochlorous acid (HOCl)/hypochlorite (OCl), an important reactive oxygen species, plays a number of important roles in various physiological processes. However, abnormal levels of OCl can cause many serious diseases, such as atherosclerosis, rheumatoid arthritis, cardiovascular disease, and cancer. The development of efficient methods to monitor OCl in biological systems is therefore of particular importance. Thus, we herein report a novel isophorone-based fluorescent probe, i.e., DCOH-FR-OCl, for OCl detection. This probe was found to exhibit colorimetric and far-red ratiometric fluorescence response signals, excellent selectivity and sensitivity for OCl (detection limit, 88.06 nM), a remarkably large Stokes shift (158 nm), an ultrafast response (completed within 3 s), perfect photostability, and good water solubility (100% in aqueous media). DCOH-FR-OCl, as we know, is the first probe that can detect OCl by using two different response signals at an ultrafast speed with a large Stokes shift in fully aqueous media. Furthermore, DCOH-FR-OCl can be successfully employed in the real-time imaging of endogenous and exogenous OCl in living cells.

Graphical abstract: A novel colorimetric and far-red emission ratiometric fluorescent probe for the highly selective and ultrafast detection of hypochlorite in water and its application in bioimaging

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Supplementary files

Article information


Submitted
11 Oct 2019
Accepted
18 Nov 2019
First published
21 Nov 2019

Analyst, 2020, Advance Article
Article type
Paper

A novel colorimetric and far-red emission ratiometric fluorescent probe for the highly selective and ultrafast detection of hypochlorite in water and its application in bioimaging

Y. Zhang, Y. Ma, Z. Wang, X. Zhang, X. Chen, S. Hou and H. Wang, Analyst, 2020, Advance Article , DOI: 10.1039/C9AN02034A

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