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A novel colorimetric and far-red emission ratiometric fluorescent probe for the highly selective and ultrafast detection of hypochlorite in water and its application in bioimaging

Abstract

Hypochlorous acid (HOCl)/hypochlorite (OCl−), important reactive oxygen species, plays a number of important roles in various physiological progresses. However, abnormal levels of OCl− can cause many serious diseases, such as atherosclerosis, rheumatoid arthritis, cardiovascular disease, and cancer. The development of efficient methods to monitor OCl− in biological systems is therefore of particular importance. Thus, we herein report a novel isophorone-based fluorescent probe, i.e., DCOH-FR-OCl, for OCl− detection. This probe was found to exhibit colorimetric and far-red ratiometric fluorescent response signals, an excellent selectivity and sensitivity for OCl− (the detection limit, 88.06 nM), a remarkably large Stokes shift (158 nm), an ultrafast response (completed within 3 s), a perfect photostability, and good water solubility (100% in aqueous media). DCOH-FR-OCl, as we know, is the first probe that can detect OCl− by two different response signals with ultrafast speed and large Stokes shift in fully aqueous media. Furthermore, DCOH-FR-OCl can be successfully employed in the real-time imaging of endogenous and exogenous OCl− of living cells.

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Publication details

The article was received on 11 Oct 2019, accepted on 18 Nov 2019 and first published on 21 Nov 2019


Article type: Paper
DOI: 10.1039/C9AN02034A
Analyst, 2019, Accepted Manuscript

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    A novel colorimetric and far-red emission ratiometric fluorescent probe for the highly selective and ultrafast detection of hypochlorite in water and its application in bioimaging

    Y. Zhang, Y. Ma, Z. Wang, X. Zhang, X. Chen, S. Hou and H. Wang, Analyst, 2019, Accepted Manuscript , DOI: 10.1039/C9AN02034A

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