Jump to main content
Jump to site search


Intramolecular photoinitiator induced atom transfer radical polymerization for DNA electrochemical detection

Abstract

A novel electrochemical biosensor was firstly reported to achieve DNA highly sensitive detection based on photoinduced atom transfer radical polymerization (photoATRP). In this work, PNA was applied as capture probes to specifically recognize the target DNA (TDNA), and we utilized lung cancer DNA as TDNA. ATRP initiator was introduced to the electrode surface via phosphate-Zr4+-carboxylate chemistry. The photoATRP was activated under the irradiation of blue light based on photoinitiator I2959, which produced free radicals via homolytic cleavage. Subsequently, Cu2+ was reduced to Cu+ under the assistance of the free radicals, and numerous electroactive probes were grafted onto the electrode surface. Under optimal conditions, limit of detection (LOD) of this method was 3.16 fM (S/N=3, R2=0.992), and the linear range was from 10 fM to 1.0 nM. More importantly, the prepared process of this biosensor was simple and less laborious with low background, suggesting a good potential in practical application.

Back to tab navigation

Supplementary files

Publication details

The article was received on 10 Oct 2019, accepted on 14 Nov 2019 and first published on 15 Nov 2019


Article type: Paper
DOI: 10.1039/C9AN02018G
Analyst, 2019, Accepted Manuscript

  •   Request permissions

    Intramolecular photoinitiator induced atom transfer radical polymerization for DNA electrochemical detection

    L. Ma, Q. Liu, L. Jian, S. Ye, X. K. Zheng and J. Kong, Analyst, 2019, Accepted Manuscript , DOI: 10.1039/C9AN02018G

Search articles by author

Spotlight

Advertisements