Novel nopinone-based fluorescent probe for colorimetric and ratiometricfor detection of hypochlorite and its application in water samples and living cells
A novel fluorescent probe (OFN) based on nopinone for detection of hypochlorite had been developed. The probe OFN exhibited a colorimetric and ratiometric response to hypochlorite with good selectivity, high sensitivity (low detection limit is 0.136 µM) and fast response time (30 s). In response to ClO−, an obviously change was observed in both the fluorescence and absorption spectra, followed with the visible color change from colorless to yellow and the fluorescence color change from yellow to green. The sensing mechanism was confirmed that the oxime group of OFN was oxidized into aldehyde group, which was proved by HRMS and 1H NMR. What’s more, the probe was not only used to detect the concentration of ClO− in water samples, but also for monitoring ClO− in living cells.