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Surface-enhanced Electrochemiluminescence Sensor Based on Au-SiO2 core-shell nanocomposites doped with Ru(bpy)32+ for Ultrasensitive Detection of Prostate specific Antigen in Human Serum

Abstract

This work reports a surface-enhanced electrochemiluminescence (SEECL) sensor for ultrasensitive detection of prostate specific antigen (PSA) in human serum. Firstly, we prepared the Au-SiO2 core-shell nanocomposites doped with Ru(bpy)32+ (Au@SiO2-Ru) as ECL signal generation and amplification element of the SEECL sensor. Ru(bpy)32+ could emit ECL under the excitation of electrochemical reaction, and generate the local surface plasmon resonance (LSPR) electromagnetic field frim the gold nanoparticle (AuNP) core. The produced LSPR would then effectively enhance the ECL signals. This sensing strategy was utilized for the detection of PSA. PSA was absorbed on the electrode surface modified with monoclonal antibody. When the addition of polyclonal antibody modified Au@SiO2-Ru, the PSA-double-antibody sandwich structure formed, thus Au@SiO2-Ru were quantitative immobilized on electrode surface. The results show that this kind of SEECL sensor has good selectivity to PSA. Notably, the minimum detection concentration could be reached 7×10-7 ng/mL under the optimum experimental conditions, which is much better than most of the previously reported approaches for the detection of PSA in serum.

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Publication details

The article was received on 28 Sep 2019, accepted on 05 Nov 2019 and first published on 06 Nov 2019


Article type: Paper
DOI: 10.1039/C9AN01935A
Analyst, 2019, Accepted Manuscript

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    Surface-enhanced Electrochemiluminescence Sensor Based on Au-SiO2 core-shell nanocomposites doped with Ru(bpy)32+ for Ultrasensitive Detection of Prostate specific Antigen in Human Serum

    D. Wang, J. Zhou, L. Guo, B. Qiu and Z. Lin, Analyst, 2019, Accepted Manuscript , DOI: 10.1039/C9AN01935A

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