Single point single-cell nanoparticle mediated pulsed laser optoporation.
In the article we present the optical platform to investigate the dynamics of individual nanoparticle assisted pulsed laser optoporation of single living cells. Here plasmonic nanoparticles (NP) act as live cell membrane spatial position markers and as an enhancer for localized pulsed laser perforation. High contrast NP imaging using reflected light microscopy (RLM) allows precise and automatic laser targeting at individual NPs for a spatially controlled laser optoporation of single cell. This RLM nanoparticles imaging method is also compatible with a cellular incubator and fluorescence microscopy for the investigation of real-time perforation kinetics of live cells and the optomechanical NP-membrane interaction. These parameters are of great interest for the development of realization of pulsed laser optoporation and transfection technique applied to single living cells analysis.