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A rhodol-hemicyanine based ratiometric fluorescent probe for real-time monitoring of glutathione dynamics in living cells

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Abstract

Glutathione (GSH) plays crucial roles in various physiological and pathological processes. The fluctuation of the GSH level is closely associated with a variety of diseases and cellular functions. Hence, it is important to real-time monitor the fluctuation of GSH in living cells. In this work, we presented a rhodol-hybridized hemicyanine fluorophore (RdH) as a selective, rapid-response, ratiometric, and reversible fluorescent probe for intracellular GSH (t1/2 = 89 ms, Kd = 1.42 mM). The imaging assays in living cells revealed that RdH could be used to real-time monitor GSH dynamics in A549 cells under a laser scanning confocal microscope by ratiometric fluorescence changes.

Graphical abstract: A rhodol-hemicyanine based ratiometric fluorescent probe for real-time monitoring of glutathione dynamics in living cells

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Publication details

The article was received on 19 Sep 2019, accepted on 30 Oct 2019 and first published on 01 Nov 2019


Article type: Paper
DOI: 10.1039/C9AN01852B
Analyst, 2019, Advance Article

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    A rhodol-hemicyanine based ratiometric fluorescent probe for real-time monitoring of glutathione dynamics in living cells

    M. Ren, L. Wang, X. Lv, Y. Sun, H. Chen, K. Zhang, Q. Wu, Y. Bai and W. Guo, Analyst, 2019, Advance Article , DOI: 10.1039/C9AN01852B

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