Jump to main content
Jump to site search


A lysosome specific theranostic NO donor inhibits cancer cells by stimuli responsive molecular self-decomposition with an on-demand fluorescence pattern

Author affiliations

Abstract

The anticancer mechanism of NO is difficult to study owing to its short lifetime and high reactivity. Thus, a theranostic anticancer NO donor assembled with NO on-demand release abilities, accurate lysosome location capabilities and signal feedback behavior was developed. Profiting from the theranostic properties, the specific mechanism was comprehensively studied. Spectral and cell imaging studies revealed that the as prepared NO donors could release NO in solution or within cancer cells. Fluorescence co-dyeing experiments demonstrated that Mo-Nap-NO entered lysosomes specifically and disrupted them after being triggered by light. Upon irradiation with 460 nm visible light, both the donors demonstrated considerable in vitro anticancer effects. A further mechanistic study showed that after entering the lysosome and being triggered by 460 nm irradiation, NO ruptured the lysosome, resulting in the release of cathepsin D into the cytosol, which activated the caspase3 mediated apoptosis pathway.

Graphical abstract: A lysosome specific theranostic NO donor inhibits cancer cells by stimuli responsive molecular self-decomposition with an on-demand fluorescence pattern

Back to tab navigation

Supplementary files

Publication details

The article was received on 05 Sep 2019, accepted on 27 Sep 2019 and first published on 30 Sep 2019


Article type: Paper
DOI: 10.1039/C9AN01746A
Analyst, 2019, Advance Article

  •   Request permissions

    A lysosome specific theranostic NO donor inhibits cancer cells by stimuli responsive molecular self-decomposition with an on-demand fluorescence pattern

    W. Hua, J. Zhao, X. Wang, S. Pei and S. Gou, Analyst, 2019, Advance Article , DOI: 10.1039/C9AN01746A

Search articles by author

Spotlight

Advertisements