Fe3O4@poly(N-Vinylcaprolactam) Grafted onto Chitosan as New Sorbent for Detection Imatinib mesylate in Biosample Followed by UPLC-MS/MS
The Fe3O4 nanoparticles with chitosan grafted onto poly(N-Vinylcaprolactam) copolymers are synthesized, which showed dual sensitivity to temperature and pH. The nanoparticles were characterized by different techniques and two aspects of their applications were evaluated using Imatinib mesylate drug as a model. The first studied application of these nanoparticles was the extraction and pre-concentration of Imatinib mesylate from biological samples. Parameters such as sorbent dosage and extraction and desorption time can affect the Imatinib mesylate extraction which were studied by Box-Behnken design and response surface methodology, while the one-factor-at-a-time approach assessed the sample pH and elution solvent type and amount. The determination and quantification were accomplished by Ultra-performance liquid chromatography–mass spectrometry. The limit of quantiﬁcation of approach was 1.0 ng.mL-1. Inter/intraday precision was obtained: 3.4–6.7% and 5.1–7.7%, respectively. Also, the modified nanoparticles were studied in basic in vitro drug delivery research which led to satisfactory results.