Detection of breast cancer-derived exosomes using the horseradish peroxidase-mimicking DNAzyme as aptasensor
Exosomes are membrane-enclosed phospholipid extracellular vesicles with a variety of tumor antigens which can be applied in the diagnosis and treatment of cancer due to the high secretion on the surface of cancer cells. Up to now, many researches on exosomes have been reported, but convenient and low-cost detection methods still need to be developed. Recently, we have developed a sensitive, simple and low-cost colorimetric aptasensor by designing a hairpin-like structure, which combined the highly specific MUC1 aptamer with a hemin/G-quadruplex for detection of breast cancer exosomes. The hemin/G-quadruplex toward H2O2 reduction were used to generate evidently strong colorimetric response owing to acting as a HRP-mimicking DNAzyme. The aptasensor is regarded as an “on-off” type switch, which strictly control the process of reaction responding to the existence or not of exosomes. In our study, associated exosome detection limits are 3.94×105 particles/mL, which showed a higher sensitivity compared to commercial ELISA. Our method not only exhibited the advantages of convenient and time-saving, with few instruments used, but also can be easily observed by naked eyes of the signal generation. Furthermore, the proposed strategy can well differentiate breast cancer patients from healthy individuals, demonstrating potential application to analysis of clinical specimens.