Two isomeric and distinguishable H2S fluorescence probes for monitoring spoilage of eggs as well as visualizing exogenous and endogenous H2S in living cells
More accurately fabricating the fluorescence probe to efficiently track and detect H2S levels in fields of foodstuffs and physiology is crucial. Herein, we reported two isomeric phenanthro[9,10-d]imidazole benzene sulfonamide-derived fluorescence probes (PI-2-SA and PI-4-SA) both of which could display remarkable response toward H2S over other analyte. The spectral characteristics of two probes were investigated and discussed in detail. By comparison, PI-2-SA was specific, sensitive (the limit of detection was ca. 12.3 nM), rapid (within ≤ 3 min) and dynamic (the rate constant was 0.02 s-1). Significantly, PI-2-SA as represent had been proved to be effective in tracking the shelf-time process of eggs samples for real time, as well as the imaging of exogenous and endogenous H2S in HeLa cells.