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Issue 20, 2019
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A feasibility study of a leaky waveguide aptasensor for thrombin

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Abstract

This proof-of-principle study demonstrates the feasibility of a leaky waveguide (LW) aptasensor, where aptamers were immobilised in a mesoporous chitosan waveguiding film for the detection of thrombin. This work has demonstrated that aptamers immobilised in hydrogels retain their affinity and selectivity towards their target and thus can be used as bioreceptors. The use of antibodies as bioreceptors for sensing thrombin is not viable because it is a serine protease, which will cleave the antibodies. Currently used assays based on clotting time and chromogenic/fluorogenic substrates have limited potential for thrombin measurement in whole blood. Using the initial binding rate over the first 5 min, the limit of detection of our LW aptasensor for thrombin was ∼22 nM. The sensor was tested with spiked serum samples, giving a reading of 46.1 ± 4.6 nM for a sample containing 50 nM thrombin. Our proposed sensor combines the robustness and low cost of aptamers as molecular recognition elements with the simple fabrication process of the chitosan-based leaky waveguide, making LW aptasensors highly attractive for applications in point-of-care diagnostics and healthcare monitoring.

Graphical abstract: A feasibility study of a leaky waveguide aptasensor for thrombin

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Article information


Submitted
27 Jul 2019
Accepted
06 Sep 2019
First published
06 Sep 2019

This article is Open Access

Analyst, 2019,144, 6048-6054
Article type
Paper

A feasibility study of a leaky waveguide aptasensor for thrombin

N. A. Alamrani, G. M. Greenway, N. Pamme, N. J. Goddard and R. Gupta, Analyst, 2019, 144, 6048
DOI: 10.1039/C9AN01421G

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