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Polymer brush based fluorescent immunosensor for direct monitoring of interleukin-1β in rat blood

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Abstract

A sandwich immunosensor was successfully developed for monitoring of interleukin-1β (IL-1β) in rat whole blood. The substrate stainless steel (SS) was first coated with a polydopamine layer and subsequently grafted with poly(ethylene glycol) methacrylate brushes, onto which a sandwich immunosensor was modified for detection of IL-1β. The device has been successfully applied for monitoring of IL-1β with a limit of detection of 4.7 pg mL−1, and a linear detection range of 12.5–200 pg mL−1. Good specificity and selectivity for monitoring of IL-1β in rat macrophage secretion were achieved. Furthermore, this device was validated by detection of IL-1β in rat whole blood samples with greater concentrations observed in obese rats compared to control, and strong positive correlation between concentrations of IL-1β and blood glucose. These results suggest this device is feasible for direct detection of target analytes in biological samples.

Graphical abstract: Polymer brush based fluorescent immunosensor for direct monitoring of interleukin-1β in rat blood

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Publication details

The article was received on 11 Jul 2019, accepted on 08 Aug 2019 and first published on 13 Aug 2019


Article type: Paper
DOI: 10.1039/C9AN01300H
Analyst, 2019, Advance Article

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    Polymer brush based fluorescent immunosensor for direct monitoring of interleukin-1β in rat blood

    F. Deng, Y. Li, M. J. Hossain, M. D. Kendig, R. Arnold, E. M. Goldys, M. J. Morris and G. Liu, Analyst, 2019, Advance Article , DOI: 10.1039/C9AN01300H

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