Jump to main content
Jump to site search


A graphene oxide-based fluorescence assay for the sensitive detection of DNA exonuclease enzymatic activity

Author affiliations

Abstract

The 3′–5′ exonuclease enzyme plays a dominant role in multiple pivotal physiological activities, such as DNA replication and repair processes. In this study, we designed a sensitive graphene oxide (GO)-based probe for the detection of exonuclease enzymatic activity. In the absence of Exo III, the strong π–π interaction between the fluorophore-tagged DNA and GO causes the efficient fluorescence quenching via a fluorescence resonance energy transfer (FRET). In contrast, in the presence of Exo III, the fluorophore-tagged 3′-hydroxyl termini of the DNA probe was digested by Exo III to set the fluorophore free from adsorption when GO was introduced, causing an inefficient fluorescence quenching. As a result, the fluorescence intensity of the sensor was found to be proportional to the concentration of Exo III; towards the detection of Exo III, this simple GO-based probe demonstrated a highly sensitive and selective linear response in the low detection range from 0.01 U mL−1 to 0.5 U mL−1 and with the limit of detection (LOD) of 0.001 U mL−1. Compared with other fluorescent probes, this assay exhibited superior sensitivity and selectivity in both buffer and fetal bovine serum samples, in addition to being cost effective and having a simple setup.

Graphical abstract: A graphene oxide-based fluorescence assay for the sensitive detection of DNA exonuclease enzymatic activity

Back to tab navigation

Publication details

The article was received on 09 Jul 2019, accepted on 06 Sep 2019 and first published on 09 Sep 2019


Article type: Paper
DOI: 10.1039/C9AN01283D
Analyst, 2019, Advance Article

  •   Request permissions

    A graphene oxide-based fluorescence assay for the sensitive detection of DNA exonuclease enzymatic activity

    X. Liu, Y. Wu, X. Wu and J. X. Zhao, Analyst, 2019, Advance Article , DOI: 10.1039/C9AN01283D

Search articles by author

Spotlight

Advertisements