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Issue 17, 2019
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Separation of isomeric glycans by ion mobility spectrometry – the impact of fluorescent labelling

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Abstract

The analysis of complex oligosaccharides is traditionally based on multidimensional workflows where liquid chromatography is coupled to tandem mass spectrometry (LC-MS/MS). Due to the presence of multiple isomers, which cannot be distinguished easily using tandem MS, a detailed structural elucidation is still challenging in many cases. Recently, ion mobility spectrometry (IMS) showed great potential as an additional structural parameter in glycan analysis. While the time-scale of the IMS separation is fully compatible to that of LC-MS-based workflows, there are very few reports in which both techniques have been directly coupled for glycan analysis. As a result, there is little knowledge on how the derivatization with fluorescent labels as common in glycan LC-MS affects the mobility and, as a result, the selectivity of IMS separations. Here, we address this problem by systematically analyzing six isomeric glycans derivatized with the most common fluorescent tags using ion mobility spectrometry. We report >150 collision cross-sections (CCS) acquired in positive and negative ion mode and compare the quality of the separation for each derivatization strategy. Our results show that isomer separation strongly depends on the chosen label, as well as on the type of adduct ion. In some cases, fluorescent labels significantly enhance peak-to-peak resolution which can help to distinguish isomeric species.

Graphical abstract: Separation of isomeric glycans by ion mobility spectrometry – the impact of fluorescent labelling

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Supplementary files

Article information


Submitted
22 May 2019
Accepted
26 Jul 2019
First published
26 Jul 2019

This article is Open Access

Analyst, 2019,144, 5292-5298
Article type
Paper

Separation of isomeric glycans by ion mobility spectrometry – the impact of fluorescent labelling

C. Manz, M. Grabarics, F. Hoberg, M. Pugini, A. Stuckmann, W. B. Struwe and K. Pagel, Analyst, 2019, 144, 5292
DOI: 10.1039/C9AN00937J

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