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Issue 17, 2019
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A high-specificity immunoassay for the therapeutic drug monitoring of cyclophosphamide

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Abstract

Personalized medicine is pushing forward new diagnostic techniques to aid in controlling drug therapeutic levels and their toxic effects. This study aims to develop a high-throughput screening method for therapeutic drug monitoring (TDM) and occupational exposure of cyclophosphamide (CP), an alkylating agent used as a chemotherapeutic and immunosuppressive drug. In order to achieve this goal, an immunizing hapten that exposes the cyclophosphamide moiety has been designed for the first time. Antibodies produced against this hapten have been used to develop an indirect competitive ELISA for the quantification of CP with high specificity and low cross-reactivity with some metabolites and other anticancer drugs. The assay obtained showed a LOD of 22 ± 6 nM in serum samples, with concentrations much below the blood CP levels of patients treated with the drug. A new tool for the detection and quantification of CP is provided which could be relevant for future pharmacokinetic studies and for therapeutic index improvement.

Graphical abstract: A high-specificity immunoassay for the therapeutic drug monitoring of cyclophosphamide

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Publication details

The article was received on 29 Mar 2019, accepted on 10 Jul 2019 and first published on 16 Jul 2019


Article type: Paper
DOI: 10.1039/C9AN00576E
Analyst, 2019,144, 5172-5178

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    A high-specificity immunoassay for the therapeutic drug monitoring of cyclophosphamide

    M. Broto, R. McCabe, R. Galve and M.-Pilar Marco, Analyst, 2019, 144, 5172
    DOI: 10.1039/C9AN00576E

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