Improvement of biosensor accuracy using an interference index detection system to minimize the interference effects caused by icterus and hemolysis in blood samples

Abstract

Reagent sensors in diagnostic assays are used in medical laboratories to obtain patient results. However, interference during the analysis of blood samples is a constant problem with reagent sensors and leads to inaccurate results. Interference in blood analysis is frequently caused by hemolysis and icterus. This study analyzed the effects of interferents on reagent sensors and devised a method to improve the measurement accuracy using an interference index detection (IID) system to minimize the interference effect. The IID system can be easily applied using only two wells and an optical component for sample measurement. After applying the IID system, the interference rates from bilirubin and hemoglobin improved dramatically. A comparison of results obtained for clinical samples showed that the IID system had a positive effect on the accuracy.