Jump to main content
Jump to site search

Issue 10, 2019
Previous Article Next Article

Monitoring mitochondrial pH with a hemicyanine-based ratiometric fluorescent probe

Author affiliations

Abstract

Mitochondria as essential organelles play critical roles in cellular metabolism. Mitochondrial pH is a vital parameter that directly affects the unique function of mitochondria. Herein, we present a new ratiometric fluorescent probe M-pH for monitoring the pH within the mitochondria. M-pH consists of a stable and large π-electron conjugated merocyanine system. The lipophilic cationic benzyl group will facilitate the accumulation of M-pH in mitochondria. The phenol unit is the recognition moiety, achieving the ratiometric sensing of pH changes. The experimental results indicate that M-pH displays ratiometric fluorescence response to different pH values. Meanwhile, M-pH shows negligible response to common species, and has high stability and low cytotoxicity. In biological experiments, M-pH can solely accumulate in mitochondria and visualize the pH changes during mitophagy and cell apoptosis. We thus believe that M-pH has great potential as a practical tool for real-time monitoring of pH changes of mitochondria, contributing to revealing the pathogenesis of mitochondrial pH associated diseases.

Graphical abstract: Monitoring mitochondrial pH with a hemicyanine-based ratiometric fluorescent probe

Back to tab navigation

Supplementary files

Publication details

The article was received on 05 Mar 2019, accepted on 11 Apr 2019 and first published on 12 Apr 2019


Article type: Paper
DOI: 10.1039/C9AN00422J
Analyst, 2019,144, 3422-3427

  •   Request permissions

    Monitoring mitochondrial pH with a hemicyanine-based ratiometric fluorescent probe

    H. Xiao, Y. Dong, J. Zhou, Z. Zhou, X. Wu, R. Wang, Z. Miao, Y. Liu and S. Zhuo, Analyst, 2019, 144, 3422
    DOI: 10.1039/C9AN00422J

Search articles by author

Spotlight

Advertisements