Jump to main content
Jump to site search

Monitoring mitochondrial pH with a hemicyanine-based ratiometric fluorescent probe


Mitochondria as the essential organelle play critical roles in cellular metabolism. Mitochondrial pH is a vital parameter, thus directly affecting the unique function of mitochondria. Herein, we present a new ratiometric fluorescent probe M-pH for monitoring the pH within mitochondria. M-pH consists of a stable and large π-electron conjugated merocyanine system. The benzyl group lipophilic cationic will facilitate the accumulation of M-pH into mitochondria. The phenol unit is the recognition moiety, achieving the ratiometric sensing of pH changes. The experimental results indicate that M-pH displays ratiometric fluorescence response to different pH values. Meanwhile, M-pH shows negligible response to common species, high stability and low cytotoxicity. In biological experiments, M-pH can solely accumulate into mitochondria and visualize the pH changes during mitophagy and cell apoptosis. We thus believe that M-pH has great potential as a practical tool for real-time monitoring of pH changes of mitochondria, contributing to revealing the pathogenesis of mitochondrial pH associated diseases.

Back to tab navigation

Supplementary files

Publication details

The article was received on 05 Mar 2019, accepted on 11 Apr 2019 and first published on 12 Apr 2019

Article type: Paper
DOI: 10.1039/C9AN00422J
Citation: Analyst, 2019, Accepted Manuscript

  •   Request permissions

    Monitoring mitochondrial pH with a hemicyanine-based ratiometric fluorescent probe

    H. Xiao, Y. Dong, J. Zhou, Z. Zhou, X. Wu, R. Wang, Z. Miao, Y. Liu and S. Zhuo, Analyst, 2019, Accepted Manuscript , DOI: 10.1039/C9AN00422J

Search articles by author