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Monitoring mitochondrial pH with a hemicyanine-based ratiometric fluorescent probe

Abstract

Mitochondria as the essential organelle play critical roles in cellular metabolism. Mitochondrial pH is a vital parameter, thus directly affecting the unique function of mitochondria. Herein, we present a new ratiometric fluorescent probe M-pH for monitoring the pH within mitochondria. M-pH consists of a stable and large π-electron conjugated merocyanine system. The benzyl group lipophilic cationic will facilitate the accumulation of M-pH into mitochondria. The phenol unit is the recognition moiety, achieving the ratiometric sensing of pH changes. The experimental results indicate that M-pH displays ratiometric fluorescence response to different pH values. Meanwhile, M-pH shows negligible response to common species, high stability and low cytotoxicity. In biological experiments, M-pH can solely accumulate into mitochondria and visualize the pH changes during mitophagy and cell apoptosis. We thus believe that M-pH has great potential as a practical tool for real-time monitoring of pH changes of mitochondria, contributing to revealing the pathogenesis of mitochondrial pH associated diseases.

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Supplementary files

Publication details

The article was received on 05 Mar 2019, accepted on 11 Apr 2019 and first published on 12 Apr 2019


Article type: Paper
DOI: 10.1039/C9AN00422J
Citation: Analyst, 2019, Accepted Manuscript

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    Monitoring mitochondrial pH with a hemicyanine-based ratiometric fluorescent probe

    H. Xiao, Y. Dong, J. Zhou, Z. Zhou, X. Wu, R. Wang, Z. Miao, Y. Liu and S. Zhuo, Analyst, 2019, Accepted Manuscript , DOI: 10.1039/C9AN00422J

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