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Highly specific and ultrasensitive probe for the imaging of inflammation-induced endogenous hypochlorous acid

Abstract

A turn-on two-photon fluorescent probe HCA-Green for hypochlorous acid (HOCl) was sythesized using 4-methylamino-1,8-naphthalimide (MNA) as the two-photon fluorophore and p-hydroxyaniline as the leaving–recognition domain. Both the probe and the fluorophore were investigated under one- and two-photon excitation modes. The fluorescence intensity of the probe was enhanced by ~229-fold and ~193-fold under one-photon and two-photon excitation, respectively, after reacting with HOCl. And a maximal two-photon action cross-section of 50 GM was obtained under excitation at 810 nm. The probe exhibited high sensitivity with a detection limit of 42.3 nM, as well as high selectivity, low cytotoxicity, and good photostability. Two-photon microscopy (TPM) was conducted to visualize HOCl levels in living cells and tissues. The production of endogeneous HOCl induced by lipopolysaccharide-mediated inflammation was successfully monitored with this probe.

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Publication details

The article was received on 27 Feb 2019, accepted on 01 Apr 2019 and first published on 11 Apr 2019


Article type: Paper
DOI: 10.1039/C9AN00390H
Citation: Analyst, 2019, Accepted Manuscript

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    Highly specific and ultrasensitive probe for the imaging of inflammation-induced endogenous hypochlorous acid

    X. Song, W. Hu, D. Wang, Z. Mao and Z. Liu, Analyst, 2019, Accepted Manuscript , DOI: 10.1039/C9AN00390H

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