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Issue 14, 2019
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Analysis of αB-crystallin polydispersity in solution through native microfluidic electrophoresis

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Abstract

In recent years, significant advancements have been made in the understanding of the population distributions and dynamic oligomeric states of the molecular chaperone αB-crystallin and its core domain variants. In this work, we provide solution-phase evidence of the polydispersity of αB-crystallin using microfluidic methods, used for separating the oligomeric species present in solution according to their different electrophoretic mobilities on-chip in a matter of seconds. We in particular demonstrate that microfluidic high-field electrophoresis and diffusion can detect the oligomerisation of these highly dynamic molecular chaperones and characterise the dominant oligomeric species present. We thereby provide a robust microfluidic method for characterising the individual species within complex protein mixtures of biological relevance.

Graphical abstract: Analysis of αB-crystallin polydispersity in solution through native microfluidic electrophoresis

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Article information


Submitted
26 Feb 2019
Accepted
02 Jun 2019
First published
19 Jun 2019

Analyst, 2019,144, 4413-4424
Article type
Paper

Analysis of αB-crystallin polydispersity in solution through native microfluidic electrophoresis

M. A. Wright, F. S. Ruggeri, K. L. Saar, P. K. Challa, J. L. P. Benesch and T. P. J. Knowles, Analyst, 2019, 144, 4413
DOI: 10.1039/C9AN00382G

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