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Issue 15, 2019
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Biotinylation and isolation of an RNA G-quadruplex based on its peroxidase-mimicking activity

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Abstract

RNA G-quadruplexes (rG4s) are important RNA secondary structures considering their significance in regulating numerous cellular processes. Described herein is an rG4 detecting and isolation method, which exploits the complex of rG4 and hemin to mimic peroxidase. In the presence of biotin tyramide and hydrogen peroxide, rG4s can be selectively self-biotinylated and easily isolated from a complex RNA mixture using streptavidin magnetic beads.

Graphical abstract: Biotinylation and isolation of an RNA G-quadruplex based on its peroxidase-mimicking activity

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Publication details

The article was received on 22 Feb 2019, accepted on 14 Jun 2019 and first published on 20 Jun 2019


Article type: Communication
DOI: 10.1039/C9AN00353C
Analyst, 2019,144, 4472-4476

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    Biotinylation and isolation of an RNA G-quadruplex based on its peroxidase-mimicking activity

    W. Li, W. Zeng, Y. Chen, F. Wang, F. Wu, X. Weng and X. Zhou, Analyst, 2019, 144, 4472
    DOI: 10.1039/C9AN00353C

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