Issue 15, 2019

Biotinylation and isolation of an RNA G-quadruplex based on its peroxidase-mimicking activity

Abstract

RNA G-quadruplexes (rG4s) are important RNA secondary structures considering their significance in regulating numerous cellular processes. Described herein is an rG4 detecting and isolation method, which exploits the complex of rG4 and hemin to mimic peroxidase. In the presence of biotin tyramide and hydrogen peroxide, rG4s can be selectively self-biotinylated and easily isolated from a complex RNA mixture using streptavidin magnetic beads.

Graphical abstract: Biotinylation and isolation of an RNA G-quadruplex based on its peroxidase-mimicking activity

Supplementary files

Article information

Article type
Communication
Submitted
22 Feb 2019
Accepted
14 Jun 2019
First published
20 Jun 2019

Analyst, 2019,144, 4472-4476

Biotinylation and isolation of an RNA G-quadruplex based on its peroxidase-mimicking activity

W. Li, W. Zeng, Y. Chen, F. Wang, F. Wu, X. Weng and X. Zhou, Analyst, 2019, 144, 4472 DOI: 10.1039/C9AN00353C

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