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Issue 10, 2019
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A highly selective and sensitive red-emitting fluorescent probe for visualization of endogenous peroxynitrite in living cells and zebrafish

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Abstract

Peroxynitrite (ONOO) has been proven to participate in various physiological and pathological processes, and may also be a contributing factor in many diseases. In view of this, there is a need to develop detection tools for unambiguously tracking a small amount of endogenous ONOO to reveal its exact mechanisms. In this paper, a colorimetric and red-emitting fluorescent probe Red-PN, based on a rhodamine-type fluorophore and hydrazide reactive site is described. The probe Red-PN possesses the advantages of rapid response (within 5 s), visual color change (from colorless to pink), preeminent sensitivity (detection limit = 4.3 nM) and selectivity. Because of these outstanding performances, it was possible to accurately detect endogenous ONOO. It was encouraging that the probe Red-PN could be used effectively for tracking the relatively low levels of endogenous and exogenous ONOO in living cells and zebrafish. Thus, it is envisioned that the probe Red-PN would have promising prospects in applications for imaging ONOO in a variety of biological settings.

Graphical abstract: A highly selective and sensitive red-emitting fluorescent probe for visualization of endogenous peroxynitrite in living cells and zebrafish

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Article information


Submitted
21 Feb 2019
Accepted
07 Apr 2019
First published
10 Apr 2019

Analyst, 2019,144, 3442-3449
Article type
Paper

A highly selective and sensitive red-emitting fluorescent probe for visualization of endogenous peroxynitrite in living cells and zebrafish

Z. Li, C. Liu, C. Yu, Y. Chen, P. Jia, H. Zhu, X. Zhang, Y. Yu, B. Zhu and W. Sheng, Analyst, 2019, 144, 3442
DOI: 10.1039/C9AN00347A

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