Ions-chelated based digital barcodes for multiplexing of suspension array
The ions-chelated microbeads (ICM) for suspension array are prepared by chelating metal ions (MIs) which are used as the encoding materials. Stimulating the ICM, laser induced breakdown spectra (LIBs) can be obtained and the atomic spectra of the chelated ions are chosen as the decoding signals. Our ICM show digital characteristics with high stability due to the property of LIBs. And since there are many available coding materials and different kinds of coding materials can be easily combined, the coding capacity can be considerably enlarged. Further, the background interference in fluoroimmunoassay detection could be avoided, because the ICM contain no fluorescence emissions. In our studies, we achieve totally 15 barcodes by taking full advantage of 4 kinds of ions, then the fluoroimmunoassay is performed to demonstrate the specificity and detection performance of our ICM in multiplexing and the detection limit could reach to 1.49×10-10M, showing promising potential in application.