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T7 exo-mediated FRET-breaking combined with DSN–RNAse–TdT for the detection of microRNA with ultrahigh signal-amplification

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Abstract

A DSN–RNAse–TdT–T7 exo probing system allows the detection of miRNA 21 with very high sensitivity (LOD = 2.57 fM) and selectivity—the result of (i) avoiding the false-positive signal from miRNA reacting with TdT polymerase and (ii) signal amplification occurring through a FRET-breaking mechanism involving T7 exo.

Graphical abstract: T7 exo-mediated FRET-breaking combined with DSN–RNAse–TdT for the detection of microRNA with ultrahigh signal-amplification

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Publication details

The article was received on 14 Feb 2019, accepted on 04 Apr 2019 and first published on 05 Apr 2019


Article type: Communication
DOI: 10.1039/C9AN00303G
Citation: Analyst, 2019, Advance Article

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    T7 exo-mediated FRET-breaking combined with DSN–RNAse–TdT for the detection of microRNA with ultrahigh signal-amplification

    V. T. Nguyen, B. H. Le and Y. J. Seo, Analyst, 2019, Advance Article , DOI: 10.1039/C9AN00303G

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