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SERS-based Rapid Assay for Sensitive Detection of Group A Streptococcus by Evaluation of the Swab Sampling Technique

Abstract

Beta-hemolytic, Group A Streptococcus pyogenes (GAS) is a life-threating pathogen and the reason of prominent disease, pharyngitis. The conventional analysis of GAS, gold standard, takes 48 hours and the related rapid tests are lack of accuracy and sensitivity. In this study, firstly, the efficiency of swab sampling, which it is a must in the GAS detection, was discussed with the proposed surface-enhanced Raman spectroscopy (SERS)-based batch assay and each step were controlled with the plate-counting method. Secondly, SERS-based lateral flow immunoassay (LFIA) test strips were also constructed and the variation in SERS intensity of 5,5′-Dithiobis (2-nitrobenzoic acid) (DTNB) was observed. Thus, a linear correlation was found with R2 of 0.9926 and the LOD was calculated as 0.2 CFU/mL of GAS which could be counted as one cell. The combination of the gold standard with the LFIA-SERS technique enabled the fast and accurate pathogen detection. In addition, GAS was quantified with paper-based test strips up to 100 CFU/mL level of bacteria for the first time without any interference. Besides, this study was featured with the discussion of the whole cell and pretreated cell detection of pathogens with LFIA. Therefore, this work enlightens the points have never been discussed on pathogen detection with paper-based platforms.

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Publication details

The article was received on 24 Jan 2019, accepted on 11 Apr 2019 and first published on 12 Apr 2019


Article type: Paper
DOI: 10.1039/C9AN00173E
Citation: Analyst, 2019, Accepted Manuscript

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    SERS-based Rapid Assay for Sensitive Detection of Group A Streptococcus by Evaluation of the Swab Sampling Technique

    M. ERYILMAZ, E. Acar Soykut, D. Cetin, I. H. Boyacı, Z. Suludere and U. Tamer, Analyst, 2019, Accepted Manuscript , DOI: 10.1039/C9AN00173E

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