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Coating DNA self-assembled monolayer with a metal organic framework-based exoskeleton for improved sensing performance

Abstract

The formation of DNA self-assembled monolayers (SAMs) is one of the most popular ways to attach DNA molecules onto solid surface, which has been extensively used in many fields, especially biosensing. However, the relatively poor stability of DNA SAMs (e.g., after a long-term storage or in harsh environmental conditions) greatly limits its use in real application. Herein, a new strategy is reported in this paper to protect the DNA SAMs by using metal organic framework (MOF)-based exoskeleton. Taking electrochemical DNA (E-DNA) sensors as example, we have systematically studied the stability of various DNA probes from the simple single-stranded DNA to complex DNA nanostructure on Au electrode surface. We have found that different DNA probes may lead to varying MOF profiles, and the formed MOFs can be conveniently removed by simple acidic water rinse. Thanks to exoskeleton, the stability of DNA SAMs is significantly enhanced and the DNA probes can be insulated from heat, nuclease, and various ion strength, greatly extending the shelf-life of E-DNA sensors and indirectly improving their sensing performance. More importantly, the secondary structure of DNA probes can also be well persevered. The longstanding stability is of particular importance to biosensors, thus they can be facilely handled, transported, and stored in the resource-limited setting without compromising the analytical performance of biosensors.

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Publication details

The article was received on 14 Jan 2019, accepted on 10 Apr 2019 and first published on 11 Apr 2019


Article type: Paper
DOI: 10.1039/C9AN00084D
Citation: Analyst, 2019, Accepted Manuscript

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    Coating DNA self-assembled monolayer with a metal organic framework-based exoskeleton for improved sensing performance

    J. Ma, W. Chai, J. Lu, T. Tian, S. Wu, Y. Yang, J. Yang, C. Li and G. Li, Analyst, 2019, Accepted Manuscript , DOI: 10.1039/C9AN00084D

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