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Issue 7, 2019
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A two-photon excited red-emissive probe for imaging mitochondria with high fidelity and its application in monitoring mitochondrial depolarization via FRET

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Abstract

Mitochondria play central roles in plenty of biological processes, such as apoptosis, signaling, and cell differentiation. Mitochondrial depolarization is a significant sign of deterioration of intracellular status. Although many fluorescent probes for visualizing mitochondria have been delivered, two-photon red-emitting mitochondrial probes capable of detecting mitochondrial depolarization remain rare. In this work, by linking a pyrene moiety to a quinoline salt, we have constructed two-photon red-emitting mitochondrial probes, MVQ and HVQ. Between them, HVQ with a longer side chain exhibits higher hydrophobic properties, and can image mitochondria with high-fidelity due to the aggregation caused quenching effect. In cooperation with MTDR, a commercialized mitochondrial probe, HVQ, could be used to image the depolarization of mitochondria induced by a protonophore and hydrogen peroxide. We believe that HVQ can serve as a powerful tool for the investigation of mitochondria and mitochondrial membrane potential in fundamental biological research.

Graphical abstract: A two-photon excited red-emissive probe for imaging mitochondria with high fidelity and its application in monitoring mitochondrial depolarization via FRET

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Publication details

The article was received on 13 Jan 2019, accepted on 07 Feb 2019 and first published on 13 Feb 2019


Article type: Paper
DOI: 10.1039/C9AN00076C
Citation: Analyst, 2019,144, 2387-2392

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    A two-photon excited red-emissive probe for imaging mitochondria with high fidelity and its application in monitoring mitochondrial depolarization via FRET

    J. Sun, M. Tian and W. Lin, Analyst, 2019, 144, 2387
    DOI: 10.1039/C9AN00076C

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