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Two-photon excited red-emissive probe for imaging mitochondria with high fidelity and its application in monitoring mitochondrial depolarization via FRET

Abstract

Mitochondria play central roles in plenty of biological processes, such as apoptosis, signaling, and cell differentiation. Mitochondrial depolarization is a signifcant sign of the deterioration of intracellular status. Although many fluorescent probes for visualizing mitochondria have been delivered, two-photon red-emitting mitchondrial probes capable of detecting mitochondrial depolarization remain rare. In this work, by linking pyrene moiety to a quinoline salt, we have constructed two-photon red-emitting mitochondrial probes, MVQ and HVQ. Amongst, HVQ with a longer side chain exhibit higher hydrophobic property, and can high-fidelity image mitochondrial due to aggregation caused quenching effect. In cooperation with MTDR, a commercialized mitochondrial probe, HVQ could be used to image the depolarization of mitochondria induced by protonophore and hydrogen peroxide. We believe that HVQ can serve as a powerful tool for investigation of mitochondria and mitochondrial membrane potential in fundamental biological researches.

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Supplementary files

Publication details

The article was received on 13 Jan 2019, accepted on 07 Feb 2019 and first published on 13 Feb 2019


Article type: Paper
DOI: 10.1039/C9AN00076C
Citation: Analyst, 2019, Accepted Manuscript

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    Two-photon excited red-emissive probe for imaging mitochondria with high fidelity and its application in monitoring mitochondrial depolarization via FRET

    J. Sun, M. Tian and W. Lin, Analyst, 2019, Accepted Manuscript , DOI: 10.1039/C9AN00076C

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