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Poly(Thymine)-Templated Fluorescent Copper Nanoparticles Hydrogel Based Visual and Portable Strategy for Organophosphorus Pesticides Assay

Abstract

Since fluorescent assay for organophosphorus pesticides (OPs) with high sensitivity is urgently required to protect ecosystem and prevent disease, the environmentally-friendly and label-free fluorescent probe is desired. Herein, poly-thymine30 DNA-templated copper nanoparticles (PolyT30-Cu NPs) hydrogel fluorescent probe was explored for the construction of the OPs sensing platform via tyrosinase (TYR) enzyme-controlled. Initially, TYR can efficiently quench the fluorescence of Poly T30-Cu NPs, but when mixing OPs with a certain amount of TYR, the fluorescence recovery of PolyT30-Cu NPs could be recovered. Base on the phenomenon, we designed a functionalized hydrogel based on PolyT30-Cu NPs for portable and visible detection of OPs with high sensitivity and selectivity. This proposed fluorescence platform was demonstrated to enable rapid detection for OPs (paraoxon as model) and to provide excellent sensitivity with a detection limit of 3.33×10-8 ng/μL and the linear range of 1.00×10-7-10.0×10-4 ng/μL. The fluorescent probe is not only low-cost without any requirement for sophisticated synthesis of organic fluorescent compound or labeling process, but also environmentally friendly due to the biotemplate of DNA and biocompatible metal of copper. Meanwhile, the use of the functional hydrogel integrates features of portability, visualization, fast signal response and environmental anti-interference making the strategy more feasible in complex practical detection.

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Publication details

The article was received on 03 Jan 2019, accepted on 05 Feb 2019 and first published on 06 Feb 2019


Article type: Paper
DOI: 10.1039/C9AN00017H
Citation: Analyst, 2019, Accepted Manuscript

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    Poly(Thymine)-Templated Fluorescent Copper Nanoparticles Hydrogel Based Visual and Portable Strategy for Organophosphorus Pesticides Assay

    J. Chen, T. Han, X. Feng, B. Wang and G. Wang, Analyst, 2019, Accepted Manuscript , DOI: 10.1039/C9AN00017H

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