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An Innovative Silicon-chip for Sensitive Real Time PCR Improvement in Pathogens Detection.

Abstract

An innovative miniaturized silicon biosensor was developed for high sensitive detection of pathogen genomes of both virus-es and bacteria trough real time PCR reaction (qRT-PCR). The device was properly designed to enhance the optical signal and performing accurate thermal control. Results show an improvement of PCR amplification by one order of magnitude in sensitivity if compared to the standard RT-PCR method. In particular for Hepatitis B virus was measured a decreasing of mean value of Ct of about 2.9 ± 0.9 compared to the standard system. Similarly, for the bacterial Pseudomonas aeruginosa, Stafilococcus Aureus and Acinetobacter baumanii the Ct decreasing was a mean values of 1.8 ± 0.5, 3.1 ± 0.5 and 3.9 ± 0.9, respectively.

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Publication details

The article was received on 02 Jan 2019, accepted on 27 Jan 2019 and first published on 06 Feb 2019


Article type: Paper
DOI: 10.1039/C9AN00006B
Citation: Analyst, 2019, Accepted Manuscript

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    An Innovative Silicon-chip for Sensitive Real Time PCR Improvement in Pathogens Detection.

    S. Battaglia, S. Petralia, N. Vicario, D. Cirillo and S. Conoci, Analyst, 2019, Accepted Manuscript , DOI: 10.1039/C9AN00006B

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