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Issue 7, 2019
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An innovative silicon-chip for sensitive real time PCR improvement in pathogen detection

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Abstract

An innovative miniaturized silicon-chip was developed for highly sensitive detection of pathogen genomes of both viruses and bacteria through real time PCR (qRT-PCR). The device was properly designed to enhance the optical signal and perform accurate thermal control. Results show an improvement of PCR amplification by one order of magnitude in sensitivity compared to the standard RT-PCR method. In particular for hepatitis B virus a decrease of the mean value of Ct of about 2.9 ± 0.9 compared to the standard system was observed. Similarly, for the bacteria Pseudomonas aeruginosa, Staphylococcus aureus and Acinetobacter baumannii, a decrease of the mean values of Ct of 1.8 ± 0.5, 3.1 ± 0.5 and 3.9 ± 0.9, respectively, was observed.

Graphical abstract: An innovative silicon-chip for sensitive real time PCR improvement in pathogen detection

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Publication details

The article was received on 02 Jan 2019, accepted on 27 Jan 2019 and first published on 06 Feb 2019


Article type: Paper
DOI: 10.1039/C9AN00006B
Citation: Analyst, 2019,144, 2353-2358

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    An innovative silicon-chip for sensitive real time PCR improvement in pathogen detection

    S. Battaglia, S. Petralia, N. Vicario, D. Cirillo and S. Conoci, Analyst, 2019, 144, 2353
    DOI: 10.1039/C9AN00006B

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