Jump to main content
Jump to site search


Direct glucose detection in whole blood by colorimetric assay based on glucose oxidase-conjugated graphene oxide/MnO2 nanozymes

Author affiliations

Abstract

We herein report a facile approach for the preparation of horseradish peroxidase (HRP)-mimic glucose oxidase-conjugated graphene oxide/MnO2 (GOD-GO/MnO2) as new nanozyme to detect the glucose concentration in whole blood. The nano-sized of MnO2 nanoparticles embedded in bovine serum albumin (BSA)-coated GO by in situ growth were evaluated focusing on the principle of HRP-mimic activity catalyzing the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide. Furthermore, we constructed dual sensing platforms based on the combination of a plasma separation pad and GOD-GO/MnO2 for direct detection of glucose concentration in whole blood by colorimetric assay without blood sample pretreatment. As a proof-of-concept, a limit of detection of 3.1 mg dL−1 for glucose was obtained with a wide linear quantification range from 25 mg dL−1 to 300 mg dL−1 through visual observation and quantitative analysis, suggesting potential clinical applications in blood glucose monitoring for diabetic patients.

Graphical abstract: Direct glucose detection in whole blood by colorimetric assay based on glucose oxidase-conjugated graphene oxide/MnO2 nanozymes

Back to tab navigation

Supplementary files

Publication details

The article was received on 16 Dec 2018, accepted on 15 Mar 2019 and first published on 19 Mar 2019


Article type: Paper
DOI: 10.1039/C8AN02440E
Citation: Analyst, 2019, Advance Article

  •   Request permissions

    Direct glucose detection in whole blood by colorimetric assay based on glucose oxidase-conjugated graphene oxide/MnO2 nanozymes

    P. Lee, N. Li, Y. Hsu, C. Peng and H. Yang, Analyst, 2019, Advance Article , DOI: 10.1039/C8AN02440E

Search articles by author

Spotlight

Advertisements