Jump to main content
Jump to site search


New method for detection of T4 polynucleotide kinase phosphatase activity through isothermal EXPonential amplification reaction

Author affiliations

Abstract

As a bifunctional enzyme, T4 polynucleotide kinase phosphatase (T4 PNKP) catalyzes the phosphorylation of 5′-hydroxyl, and also removes the terminal 3′-phosphate group. This is closely related to the restructuring, replication, and damage repair of nucleic acid. In this paper, we describe a new method for the sensitive detection of T4 PNKP activity based on the isothermal EXPonential amplification reaction (EXPAR). T4 PNKP can be linearly assayed in the range from 0.001 to 0.01 U mL−1 with a detection limit of 7.9 × 10−4 U mL−1. Moreover, the method exhibits high specificity and sensitivity and can be applied in the enzyme analysis of complex serum samples. In view of its simplicity and moderate experimental conditions, the method may suitable for use in a commercial kit for the analysis of T4 PNKP activity.

Graphical abstract: New method for detection of T4 polynucleotide kinase phosphatase activity through isothermal EXPonential amplification reaction

Back to tab navigation

Supplementary files

Publication details

The article was received on 06 Dec 2018, accepted on 21 Jan 2019 and first published on 22 Jan 2019


Article type: Paper
DOI: 10.1039/C8AN02368A
Citation: Analyst, 2019, Advance Article

  •   Request permissions

    New method for detection of T4 polynucleotide kinase phosphatase activity through isothermal EXPonential amplification reaction

    H. Chen, Z. Wang, X. Chen, K. Lou, A. Sheng, T. Chen, G. Chen and J. Zhang, Analyst, 2019, Advance Article , DOI: 10.1039/C8AN02368A

Search articles by author

Spotlight

Advertisements