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Rapid and Sensitive Detection of the Activity of ADAM17 using Graphene Oxide-based Fluorescent Sensor

Abstract

A disintegrin and metalloproteinase 17 (ADAM17) has become a novel biomarker and potential therapeutic target for the early detection and treatment of human cancers. In this work, by covalently attaching fluorescent labeled ADAM17 substrate peptide (Pep-FAM) molecules to carboxylated graphene oxide (cGO), and monitoring the cleavage of the peptide substrate by ADAM17, we developed a cGO-Pep-FAM fluorescent sensor for the rapid, sensitive and accurate detection of ADAM17. The sensor was highly sensitive with a detection limit of 17.5 picomolar. Furthermore, the sensor was selective: structure similar proteases such as ADAM9 and MMP-9 would not interfere with ADAM17 detection. In addition, simulated serum samples were successfully analyzed. Our developed cGO-Pep-FAM sensing strategy should find useful applications in disease diagnosis and drug screening.

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Publication details

The article was received on 03 Dec 2018, accepted on 09 Jan 2019 and first published on 11 Jan 2019


Article type: Paper
DOI: 10.1039/C8AN02344A
Citation: Analyst, 2019, Accepted Manuscript
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    Rapid and Sensitive Detection of the Activity of ADAM17 using Graphene Oxide-based Fluorescent Sensor

    Y. Zhang, X. Chen, G. M. Roozbahani and X. Guan, Analyst, 2019, Accepted Manuscript , DOI: 10.1039/C8AN02344A

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