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Issue 6, 2019
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Microfluidic MeDIP-seq for low-input methylomic analysis of mammary tumorigenesis in mice

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Abstract

Studies of dynamic epigenomic changes during tumorigenesis using mice often require profiling epigenomes using a tiny quantity of tissue samples. Conventional epigenomic tests do not support such analysis due to the large amount of materials required by these assays. In this study, we developed an ultrasensitive microfluidics-based methylated DNA immunoprecipitation followed by next-generation sequencing (MeDIP-seq) technology for profiling methylomes using as little as 0.5 ng DNA (or ∼100 cells) with 1.5 h on-chip process for immunoprecipitation. This technology enabled us to examine genome-wide DNA methylation in a C3(1)/SV40 T-antigen transgenic mouse model during different stages of mammary cancer development. Using our data, we identified differentially methylated regions and their associated genes in different periods of cancer development. Our results showed that unique methylomic features were presented in various tumor developmental stages.

Graphical abstract: Microfluidic MeDIP-seq for low-input methylomic analysis of mammary tumorigenesis in mice

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Publication details

The article was received on 22 Nov 2018, accepted on 30 Dec 2018 and first published on 03 Jan 2019


Article type: Paper
DOI: 10.1039/C8AN02271B
Citation: Analyst, 2019,144, 1904-1915

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    Microfluidic MeDIP-seq for low-input methylomic analysis of mammary tumorigenesis in mice

    Y. Zhu, Z. Cao and C. Lu, Analyst, 2019, 144, 1904
    DOI: 10.1039/C8AN02271B

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