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Oligomer based real-time detection of microorganisms producing nuclease enzymes

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Abstract

In this study, we provide a method using fluorescently labeled oligonucleotides for the diagnosis of microorganisms producing nucleases in real time, while growing them in culture media. The detection of such microorganisms was possible in a short period of time, as short as 10 minutes up to a maximum of 8 hours, depending on the bacterial density. We also showed the suitability of this new method for determination of minimum inhibitory concentration (MIC) in culture media in a very short period of time, compared to conventional methods. We believe that it can make a significant contribution to gain new insights for analysis of complex materials such as clinical samples, food samples and environmental samples.

Graphical abstract: Oligomer based real-time detection of microorganisms producing nuclease enzymes

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Publication details

The article was received on 04 Nov 2018, accepted on 11 Dec 2018 and first published on 20 Dec 2018


Article type: Paper
DOI: 10.1039/C8AN02129E
Citation: Analyst, 2019, Advance Article
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    Oligomer based real-time detection of microorganisms producing nuclease enzymes

    E. Mozioğlu, S. Akyürek, S. Gündüz, M. Akgoz, A. C. Gören and T. Kocagöz, Analyst, 2019, Advance Article , DOI: 10.1039/C8AN02129E

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