Jump to main content
Jump to site search


Simultaneous quantification of multiple bacterial metabolites using surface-enhanced Raman scattering

Author affiliations

Abstract

Given the commercial importance of the compounds produced by genetically modified organisms, there is a need for screening methods which facilitate the evaluation of newly developed strains, especially during the phase of proof-of-concept development. We report a time-efficient analysis method for the screening of bacterial strains, which enables the detection of two structurally similar secondary bacterial metabolites. By combining liquid–liquid extraction and surface-enhanced Raman scattering we were able to quantify p-coumaric acid and cinnamic acid, produced by genetically modified E. coli from tyrosine and phenylalanine, respectively. With the simple sample pre-treatment method, and by applying a partial least squares data analysis method, we simultaneously detected the analytes from four E. coli strains cultured in the presence or absence of tyrosine and phenylalanine.

Graphical abstract: Simultaneous quantification of multiple bacterial metabolites using surface-enhanced Raman scattering

Back to tab navigation

Supplementary files

Publication details

The article was received on 04 Nov 2018, accepted on 28 Dec 2018 and first published on 04 Jan 2019


Article type: Paper
DOI: 10.1039/C8AN02128G
Citation: Analyst, 2019, Advance Article
  •   Request permissions

    Simultaneous quantification of multiple bacterial metabolites using surface-enhanced Raman scattering

    L. Morelli, F. A. Centorbi, O. Ilchenko, C. B. Jendresen, D. Demarchi, A. T. Nielsen, K. Zór and A. Boisen, Analyst, 2019, Advance Article , DOI: 10.1039/C8AN02128G

Search articles by author

Spotlight

Advertisements