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Aptamer superstructure-based electrochemical biosensor for sensitive detection of ATP in rat brain with in vivo microdialysis

Abstract

Highly sensitive and selective sensing for ATP in rat brain has attracted increasing interest from interdisciplinary fields of analytical chemistry and neuroscience owing to the great importance of ATP in cellular metabolism and signal transduction. Herein, we demonstrate an electrochemical biosensor for selective and sensitive detection of ATP in rat brain with aptamer superstructure as a recognition element. Unlike the electrochemical aptamer-based sensors (aptasensors) built by assembling a simple DNA structure containing only one aptamer unit onto electrode substrate, the aptasensor described here is developed by assembling aptamer superstructure, consisting of consecutive aptamer units in DNA strands, onto electrode substrate. Each aptamer unit in the superstructure is labelled with an electrochemical probe (i.e., methylene blue, MB) for signal readout. The aptamer superstructure is assembled onto gold electrode surface to form the electrochemical aptasensor. With the presence of ATP, the strong electrochemical signals resulted from the multiple redox molecules labeled on the aptamer units are clearly decreased because of the disassembling of aptamer superstructure from the electrode surface due to the strong interaction between ATP and the aptamer units. In such an approach, the aptasensor is well responsive to the ATP concentration with the current decrease being linear with the ATP concentration ranging from 0.1 nM to 1 mM. Moreover, the aptasensor has a high selectivity and good regenerability. These properties eventually enable the aptasensor with aptamer superstructure for practical application for ATP assay in rat brain combined with in vivo microdialysis.

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Publication details

The article was received on 29 Oct 2018, accepted on 31 Dec 2018 and first published on 03 Jan 2019


Article type: Paper
DOI: 10.1039/C8AN02077A
Citation: Analyst, 2019, Accepted Manuscript
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    Aptamer superstructure-based electrochemical biosensor for sensitive detection of ATP in rat brain with in vivo microdialysis

    Y. Jiang, W. Ma, W. Ji, H. Wei and L. Mao, Analyst, 2019, Accepted Manuscript , DOI: 10.1039/C8AN02077A

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