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Single particle ICP-MS-based absolute and relative quantification of E. coli O157 16S rRNA using sandwich hybridization capture

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Abstract

Herein, a novel 16S rRNA detection platform was achieved by combining a sandwich hybridization reaction, a single-molecule magnetic capture, and single particle-inductively coupled plasma mass spectrometry amplification. The assay was developed for the direct detection of RNA from dangerous human pathogens and enabled absolute and high-precision quantification of a target with a detection limit of 10 fM.

Graphical abstract: Single particle ICP-MS-based absolute and relative quantification of E. coli O157 16S rRNA using sandwich hybridization capture

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Publication details

The article was received on 26 Oct 2018, accepted on 28 Dec 2018 and first published on 03 Jan 2019


Article type: Paper
DOI: 10.1039/C8AN02063A
Citation: Analyst, 2019, Advance Article
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    Single particle ICP-MS-based absolute and relative quantification of E. coli O157 16S rRNA using sandwich hybridization capture

    X. Xu, J. Chen, B. Li, L. Tang and J. Jiang, Analyst, 2019, Advance Article , DOI: 10.1039/C8AN02063A

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