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A highly sensitive electrochemical aptasensor for detection of microcystin-LR based on a dual signal amplification strategy

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Abstract

In this work, a sensitive and selective electrochemical aptasensor for determination of microcystin-LR (MC-LR) was developed based on a dual signal amplification system consisting of a novel ternary composite and horseradish peroxidase (HRP). The ternary composite was prepared by depositing gold nanoparticles (AuNPs) on molybdenum disulfide (MoS2) covered TiO2 nanobeads (TiONBs). MoS2 nanosheet modified TiONBs provided a large surface area for immobilization of AuNPs and biomolecules. The ternary composite also possesses an improved electron transfer and catalytic capability. To construct the aptasensor, thiolated MC-LR aptamers were immobilized on the AuNP@MoS2-TiONB modified electrode through a gold–sulfur bond. Then, biotin–cDNA with a sequence complementary to the MC-LR aptamer competed with MC-LR for binding to the immobilized aptamer. The current signal catalyzed by avidin–HRP decreased with the increase of MC-LR, based on which a linear range of 0.005–30 nM and a detection limit of 0.002 nM were obtained.

Graphical abstract: A highly sensitive electrochemical aptasensor for detection of microcystin-LR based on a dual signal amplification strategy

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Publication details

The article was received on 14 Oct 2018, accepted on 31 Dec 2018 and first published on 03 Jan 2019


Article type: Paper
DOI: 10.1039/C8AN01971A
Citation: Analyst, 2019, Advance Article
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    A highly sensitive electrochemical aptasensor for detection of microcystin-LR based on a dual signal amplification strategy

    X. Liu, Y. Tang, P. Liu, L. Yang, L. Li, Q. Zhang, Y. Zhou and Md. Z. H. Khan, Analyst, 2019, Advance Article , DOI: 10.1039/C8AN01971A

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