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Study of Peak Capacities Generated by a Porous Layered Radially Elongated Pillar Array Column Coupled to a Nano-LC System

Abstract

The performance of a porous-layered radially elongated pillar (PLREP) array column in a commercial nano-LC system was examined by performing separations of alkylphenones and peptides. The mesoporous silica layer was prepared by sol-gel processing of a mixture of tetramethoxysilane and metyltrimethoxysilane on REPs filling 16.5 cm long, 1 mm wide channel (three lanes of 5.5 cm long channels connected by turns). The minimum plate height of 1.4 µm for octanophenone (k = 2.21) observed in isocratic mode is 5 times smaller than the smallest off-column plate height previously reported for porous pillar array columns for a retained component. This advantage is related to the earlier introduced shape of the radially elongated pillar bed that outperforms the cylindrically shaped pillar bed in terms of plate height. In gradient mode, maximum conditional peak capacities of 220 (for a mixture of thiourea and 7 alkylphenones, tG = 180 min) and 160 (for cytochrome c digest, tG = 150 min) were obtained. These results indicate an excellent potential for implementation of this sol-gel layer in pillar array column formats.

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Publication details

The article was received on 09 Oct 2018, accepted on 31 Dec 2018 and first published on 08 Jan 2019


Article type: Paper
DOI: 10.1039/C8AN01937A
Citation: Analyst, 2019, Accepted Manuscript
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    Study of Peak Capacities Generated by a Porous Layered Radially Elongated Pillar Array Column Coupled to a Nano-LC System

    S. Futagami, T. Hara, H. Ottevaere, H. Terryn, G. V. Baron, G. Desmet and W. De Malsche, Analyst, 2019, Accepted Manuscript , DOI: 10.1039/C8AN01937A

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