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Two-photon imaging of the endoplasmic reticulum thiol flux in the brains of mice with depression phenotypes

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Abstract

Depression is a common mental illness with high morbidity and mortality. Mounting evidence suggests that an imbalance of the oxidant–antioxidant defence system is strongly correlated with depression and the dysfunction of the endoplasmic reticulum (ER) is strongly related to the oxidative stress. Therefore, as vital and abundant antioxidants in the ER, biothiols may contribute to the etiology of depression. However, ideal two-photon (TP) fluorescent probes for in vivo imaging of ER-associated thiols in the brains of mice with depression phenotypes are still lacking. Hence, we describe a fluorescent probe (ER-SH) to visualize thiols in living systems. ER-SH displays high sensitivity, excellent ER-targeting ability, outstanding TP properties and low cytotoxicity. Using this ER-SH probe, we succeeded in revealing an increase in the endogenous thiol levels under ER stress induced by DTT. Significantly, TP in vivo imaging showed for the first time that the thiol levels are reduced in brains of mice with depression phenotypes. Collectively, this work can assist in further understanding the molecular mechanism of depression and offers a crucial dimension for diagnosis and anti-depression treatments.

Graphical abstract: Two-photon imaging of the endoplasmic reticulum thiol flux in the brains of mice with depression phenotypes

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Publication details

The article was received on 21 Aug 2018, accepted on 22 Oct 2018 and first published on 23 Oct 2018


Article type: Paper
DOI: 10.1039/C8AN01626G
Citation: Analyst, 2019, Advance Article
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    Two-photon imaging of the endoplasmic reticulum thiol flux in the brains of mice with depression phenotypes

    P. Li, X. Shi, H. Xiao, Q. Ding, X. Bai, C. Wu, W. Zhang and B. Tang, Analyst, 2019, Advance Article , DOI: 10.1039/C8AN01626G

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