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Quantitative imaging of lipid droplets in single cells

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Abstract

The combination of next generation sequencing (NGS) and automated liquid handling platforms has led to a revolution in single-cell genomic studies. However, many molecules that are critical to understanding the functional roles of cells in a complex tissue or organs, are not directly encoded in the genome, and therefore cannot be profiled with NGS. Lipids, for example, play a critical role in many metabolic processes but cannot be detected by sequencing. Recent developments in quantitative imaging, particularly coherent Raman scattering (CRS) techniques, have produced a suite of tools for studying lipid content in single cells. This article reviews CRS imaging and computational image processing techniques for non-destructive profiling of dynamic changes in lipid composition and spatial distribution at the single-cell level. As quantitative CRS imaging progresses synergistically with microfluidic and microscopic platforms for single-cell genomic analysis, we anticipate that these techniques will bring researchers closer towards combined lipidomic and genomic analysis.

Graphical abstract: Quantitative imaging of lipid droplets in single cells

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Publication details

The article was received on 08 Aug 2018, accepted on 17 Oct 2018 and first published on 17 Oct 2018


Article type: Minireview
DOI: 10.1039/C8AN01525B
Citation: Analyst, 2019, Advance Article
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    Quantitative imaging of lipid droplets in single cells

    A. Gupta, G. F. Dorlhiac and A. M. Streets, Analyst, 2019, Advance Article , DOI: 10.1039/C8AN01525B

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