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A near-infrared fluorescent probe for monitoring leucine aminopeptidase in living cells

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Abstract

Leucine aminopeptidase (LAP), one of the important cancer-related biomarkers, is significantly over-expressed in many malignant tumor cells. Developing an effective fluorescent probe for high-specificity and in situ trapping of endogenous LAP in living samples is still challenging. In this project, we report a water-soluble near-infrared (NIR) fluorescent probe (CHMC-M-Leu) for specific monitoring of LAP in vitro and in vivo. The novel fluorescent probe (CHMC-M-Leu) contains a NIR-emitting fluorophore (CHMC-M) as the reporter and L-leucine as the enzyme-active trigger moiety which are linked together by a p-aminobenzyl alcohol (PABA) section. Upon exposure to LAP, the fluorescence at 625 nm gets impressively enhanced, which belongs to the near-infrared region and is beneficial for imaging in vivo. Furthermore, the novel fluorescent probe exhibits fast response and highly chemoselective detection of LAP in various bio-related species. In addition, CHMC-M-Leu shows favourable cellular uptake and was successfully used to monitor endogenous LAP in living cells.

Graphical abstract: A near-infrared fluorescent probe for monitoring leucine aminopeptidase in living cells

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Publication details

The article was received on 02 Aug 2018, accepted on 11 Oct 2018 and first published on 12 Oct 2018


Article type: Paper
DOI: 10.1039/C8AN01486H
Citation: Analyst, 2019, Advance Article
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    A near-infrared fluorescent probe for monitoring leucine aminopeptidase in living cells

    Y. Chai, Y. Gao, H. Xiong, W. Lv, G. Yang, C. Lu, J. Nie, C. Ma, Z. Chen, J. Ren and F. Wang, Analyst, 2019, Advance Article , DOI: 10.1039/C8AN01486H

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