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Issue 47, 2019
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Colloidal crystals of compliant microgel beads to study cell migration and mechanosensitivity in 3D

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Abstract

Tissues are defined not only by their biochemical composition, but also by their distinct mechanical properties. It is now widely accepted that cells sense their mechanical environment and respond to it. However, studying the effects of mechanics in in vitro 3D environments is challenging since current 3D hydrogel assays convolve mechanics with gel porosity and adhesion. Here, we present novel colloidal crystals as modular 3D scaffolds where these parameters are principally decoupled by using monodisperse, protein-coated PAAm microgel beads as building blocks, so that variable stiffness regions can be achieved within one 3D colloidal crystal. Characterization of the colloidal crystal and oxygen diffusion simulations suggested the suitability of the scaffold to support cell survival and growth. This was confirmed by live-cell imaging and fibroblast culture over a period of four days. Moreover, we demonstrate unambiguous durotactic fibroblast migration and mechanosensitive neurite outgrowth of dorsal root ganglion neurons in 3D. This modular approach of assembling 3D scaffolds from mechanically and biochemically well-defined building blocks allows the spatial patterning of stiffness decoupled from porosity and adhesion sites in principle and provides a platform to investigate mechanosensitivity in 3D environments approximating tissues in vitro.

Graphical abstract: Colloidal crystals of compliant microgel beads to study cell migration and mechanosensitivity in 3D

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Publication details

The article was received on 19 Jun 2019, accepted on 30 Oct 2019 and first published on 07 Nov 2019


Article type: Paper
DOI: 10.1039/C9SM01226E
Soft Matter, 2019,15, 9776-9787
  • Open access: Creative Commons BY license
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    Colloidal crystals of compliant microgel beads to study cell migration and mechanosensitivity in 3D

    K. Wagner, S. Girardo, R. Goswami, G. Rosso, E. Ulbricht, P. Müller, D. Soteriou, N. Träber and J. Guck, Soft Matter, 2019, 15, 9776
    DOI: 10.1039/C9SM01226E

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