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Issue 24, 2019
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Combined molecular/continuum modeling reveals the role of friction during fast unfolding of coiled-coil proteins

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Abstract

Coiled-coils are filamentous proteins that form the basic building block of important force-bearing cellular elements, such as intermediate filaments and myosin motors. In addition to their biological importance, coiled-coil proteins are increasingly used in new biomaterials including fibers, nanotubes, or hydrogels. Coiled-coils undergo a structural transition from an α-helical coil to an unfolded state upon extension, which allows them to sustain large strains and is critical for their biological function. By performing equilibrium and out-of-equilibrium all-atom molecular dynamics (MD) simulations of coiled-coils in explicit solvent, we show that two-state models based on Kramers' or Bell's theories fail to predict the rate of unfolding at high pulling rates. We further show that an atomistically informed continuum rod model accounting for phase transformations and for the hydrodynamic interactions with the solvent can reconcile two-state models with our MD results. Our results show that frictional forces, usually neglected in theories of fibrous protein unfolding, reduce the thermodynamic force acting on the interface, and thus control the dynamics of unfolding at different pulling rates. Our results may help interpret MD simulations at high pulling rates, and could be pertinent to cytoskeletal networks or protein-based artificial materials subjected to shocks or blasts.

Graphical abstract: Combined molecular/continuum modeling reveals the role of friction during fast unfolding of coiled-coil proteins

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Publication details

The article was received on 17 Jan 2019, accepted on 24 May 2019 and first published on 30 May 2019


Article type: Paper
DOI: 10.1039/C9SM00117D
Soft Matter, 2019,15, 4961-4975

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    Combined molecular/continuum modeling reveals the role of friction during fast unfolding of coiled-coil proteins

    A. Torres-Sánchez, J. M. Vanegas, P. K. Purohit and M. Arroyo, Soft Matter, 2019, 15, 4961
    DOI: 10.1039/C9SM00117D

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