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Issue 14, 2019
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Switching transcription with bacterial RNA polymerase through photocaging, photorelease and phosphorylation reactions in the major groove of DNA

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Abstract

We report proof of principle biomimetic switching of transcription in vitro through non-natural chemical reactions in the major groove of DNA templates. Photocaged DNA templates containing nitrobenzyl-protected 5-hydroxymethyluracil or – cytosine permitted no transcription with E. coli RNA polymerase (OFF state). Their irradiation with 400 nm light resulted in DNA templates containing hydroxymethylpyrimidines, which switched transcription ON with a higher yield (250–350%) compared to non-modified DNA. Phosphorylation of templates containing 5-hydroxymethyluracil (but not 5-hydroxymethylcytosine) then turned transcription OFF again. It is the first step towards artificial bioorthogonal chemical epigenetics.

Graphical abstract: Switching transcription with bacterial RNA polymerase through photocaging, photorelease and phosphorylation reactions in the major groove of DNA

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Publication details

The article was received on 14 Jan 2019, accepted on 01 Mar 2019 and first published on 04 Mar 2019


Article type: Edge Article
DOI: 10.1039/C9SC00205G
Chem. Sci., 2019,10, 3937-3942
  • Open access: Creative Commons BY license
    All publication charges for this article have been paid for by the Royal Society of Chemistry

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    Switching transcription with bacterial RNA polymerase through photocaging, photorelease and phosphorylation reactions in the major groove of DNA

    Z. Vaníková, M. Janoušková, M. Kambová, L. Krásný and M. Hocek, Chem. Sci., 2019, 10, 3937
    DOI: 10.1039/C9SC00205G

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