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Designer artificial membrane binding proteins to direct stem cells to the myocardium

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Abstract

We present a new cell membrane modification methodology where the inherent heart tissue homing properties of the infectious bacteria Streptococcus gordonii are transferred to human stem cells. This is achieved via the rational design of a chimeric protein–polymer surfactant cell membrane binding construct, comprising the cardiac fibronectin (Fn) binding domain of the bacterial adhesin protein CshA fused to a supercharged protein. Significantly, the protein–polymer surfactant hybrid spontaneously inserts into the plasma membrane of stem cells without cytotoxicity, instilling the cells with a high affinity for immobilized fibronectin. Moreover, we show that this cell membrane reengineering approach significantly improves retention and homing of stem cells delivered either intracardially or intravenously to the myocardium in a mouse model.

Graphical abstract: Designer artificial membrane binding proteins to direct stem cells to the myocardium

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Publication details

The article was received on 31 May 2019, accepted on 07 Jun 2019 and first published on 03 Jul 2019


Article type: Edge Article
DOI: 10.1039/C9SC02650A
Chem. Sci., 2019, Advance Article
  • Open access: Creative Commons BY-NC license
    All publication charges for this article have been paid for by the Royal Society of Chemistry

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    Designer artificial membrane binding proteins to direct stem cells to the myocardium

    W. Xiao, T. I. P. Green, X. Liang, R. C. Delint, G. Perry, M. S. Roberts, K. Le Vay, C. R. Back, R. Ascione, H. Wang, P. R. Race and A. W. Perriman, Chem. Sci., 2019, Advance Article , DOI: 10.1039/C9SC02650A

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