Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 37, 2019
Previous Article Next Article

A conceptual framework for the development of iridium(iii) complex-based electrogenerated chemiluminescence labels

Author affiliations

Abstract

Translation of the highly promising electrogenerated chemiluminescence (ECL) properties of Ir(III) complexes (with tri-n-propylamine (TPrA) as a co-reactant) into a new generation of ECL labels for ligand binding assays necessitates the introduction of functionality suitable for bioconjugation. Modification of the ligands, however, can affect not only the photophysical and electrochemical properties of the complex, but also the reaction pathways available to generate light. Through a combined theoretical and experimental study, we reveal the limitations of conventional approaches to the design of electrochemiluminophores and introduce a new class of ECL label, [Ir(C^N)2(pt-TOxT-Sq)]+ (where C^N is a range of possible cyclometalating ligands, and pt-TOxT-Sq is a pyridyltriazole ligand with trioxatridecane chain and squarate amide ethyl ester), which outperformed commercial Ir(III) complex labels in two commonly used assay formats. Predicted limits on the redox potentials and emission wavelengths of Ir(III) complexes capable of generating ECL via the dominant pathway applicable in microbead supported ECL assays were experimentally verified by measuring the ECL intensities of the parent luminophores at different applied potentials, and comparing the ECL responses for the corresponding labels under assay conditions. This study provides a framework to tailor ECL labels for specific assay conditions and a fundamental understanding of the ECL pathways that will underpin exploration of new luminophores and co-reactants.

Graphical abstract: A conceptual framework for the development of iridium(iii) complex-based electrogenerated chemiluminescence labels

Back to tab navigation

Supplementary files

Article information


Submitted
21 Mar 2019
Accepted
26 Jul 2019
First published
31 Jul 2019

This article is Open Access
All publication charges for this article have been paid for by the Royal Society of Chemistry

Chem. Sci., 2019,10, 8654-8667
Article type
Edge Article

A conceptual framework for the development of iridium(III) complex-based electrogenerated chemiluminescence labels

L. Chen, D. J. Hayne, E. H. Doeven, J. Agugiaro, D. J. D. Wilson, L. C. Henderson, T. U. Connell, Y. H. Nai, R. Alexander, S. Carrara, C. F. Hogan, P. S. Donnelly and P. S. Francis, Chem. Sci., 2019, 10, 8654
DOI: 10.1039/C9SC01391A

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material and it is not used for commercial purposes.

Reproduced material should be attributed as follows:

  • For reproduction of material from NJC:
    [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
  • For reproduction of material from PCCP:
    [Original citation] - Published by the PCCP Owner Societies.
  • For reproduction of material from PPS:
    [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
  • For reproduction of material from all other RSC journals:
    [Original citation] - Published by The Royal Society of Chemistry.

Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.


Social activity

Search articles by author

Spotlight

Advertisements