Issue 2, 2019

Nuclear singlet multimers (NUSIMERs) with long-lived singlet states


Magnetic resonance (NMR) is a powerful tool in chemical analysis, structure determination and in medical diagnostics. Developing novel biological sensors for this field holds promise to better investigate protein structures or target diseases more efficiently. Herein, we explore nuclear spin singlet states in dendritic macromolecules as a platform molecule to develop stimuli responsive probes. We have developed a nuclear singlet multimer (NUSIMER) based on a generation 5 poly(amidoamine) dendrimer (PAMAM) which contains on average about 90 accessible nuclear spin singlet states with lifetimes up to 10-fold longer than the T1 relaxation times (up to 10 seconds Tsvs. T1 < 0.5 seconds) in a single molecule. We demonstrate little influence on the singlet lifetime in phosphate buffer (H2O) and a high viscosity gel environment in the presence of paramagnetic oxygen. Additionally, we demonstrate an increase in singlet lifetime upon the release of a protective chemical moiety from the NUSIMER following a stimulus, whereby no change in longitudinal relaxation time is observed. The robustness and change in singlet lifetime of the NUSIMER holds promise for the development of a novel type of biosensors.

Graphical abstract: Nuclear singlet multimers (NUSIMERs) with long-lived singlet states

Supplementary files

Article information

Article type
Edge Article
27 Jun 2018
24 Oct 2018
First published
25 Oct 2018
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY license

Chem. Sci., 2019,10, 413-417

Nuclear singlet multimers (NUSIMERs) with long-lived singlet states

P. Saul, S. Mamone and S. Glöggler, Chem. Sci., 2019, 10, 413 DOI: 10.1039/C8SC02831A

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